The NIH Neurogenomics Project at Northwestern University
The NIH Neurogenomics Project at Northwestern University, directed by
Dr. Joseph S. Takahashi, conducts a genome-wide, phenotype-driven, N-ethyl-N-nitrosourea (ENU)
mutagenesis screen for mouse mutations.
The NIH Neurogenomics Project collaborates with The
Neuroscience Mutagenesis Facility at The Jackson Laboratory and the
Neuromutagenesis Project of the Tennessee Mouse Genome Consortium to form a consortium
known as Neuromice.org.
This consortium has a collective purpose to detect, characterize and distribute new mouse mutants
with neurological and behavioral phenotypes.
Mouse Line Highlights
More lines can be found at Neuromice.org.
||Mode of Inheritance
||A-wave of electroretinogram (ERG) is almost twice the normal peak amplitude at 12 weeks
of age. Other waves are normal.
||Increased body mass as compared to a normal mouse; longer body length as compared to normal mouse
||Mutant animals lean to one side. Some circling behavior is observed, but is not pronounced.
Hearing appears unaffected.
||All waves of ERG are undetectable or very small at 12 weeks of age.
||Free running period approximately one hour shorter than wild-type mice
Mutagenesis Breeding and Phenotyping
Genome-wide mutagenesis is accomplished with the chemical ENU.
A three-generation breeding scheme is used to produce mice homozygous for induced
mutations. To date, >13,000 mice have been produced and phenotypically screened. Production and screening are continuing.
Mice are tested in a series of phenotypic screens focused on 5 domains.
An additional set of supplemental assays comprise a “preliminary assessment.”